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Statistical analysis In the in vitro alkaline comet assay, significant differences between control and treated cells were evaluated using the unpaired Welchs t -test, one-tailed (PCLiS, PHH and HepG2 cells), as equal variance was not assumed, or paired t -test, one-tailed (PRH, due to variability of each rat), using TIBCOs Statistica (StatSoft GmbH, Germany)

The veins have a bulging, lumpy or twisted appearance and are usually purple or dark blue

Excess urine calcium increases the risk of calcium oxalate and calcium phosphate kidney stones (Escribano 2014)

Other people may experience a worsening of their overall mental health