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Analytical Chemistry , 93 (4), 20722081

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Protocol [2] Rats: Serial dilutions of dihexa in 50% DMSO or water (for dilutions of 50 g/mL or less) are prepared from the stock used to dose the animals to be used for preparation of a standard curve

To correlate lux-based bioluminescence and bacterial metabolic activity, stationary-phase JE2 and JE2-lux cultures were subcultured at 1:200 in MHB, incubated at 37 C for 3 h with shaking, then treated with 00.5 mM sodium arsenate (Sigma) for 30 min