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Although general anesthesia is not required, we recommend administering Valium IV and using nitrous oxide to help alleviate any pain or discomfort during the procedure

Molecular Metabolism, 46 , Article 101090

The brain functional tissue, known as brain parenchyma, is composed of neurons and glial cells (astrocytes, oligodendrocytes, and microglia), and like every organ, it gets nutrients and oxygen from the bloodstream