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The percentage of cells with immunostained MT was determined by an automated image analyzer (Axiocam, Zeiss) after total and MT-stain-positive cells were counted at 100 magnification in three random fields (90 nm 2 ) of three different animals

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Several sub-cellular compartments generating ROS have been identified, including cytosol, peroxisomes, plasma, and endoplasmic reticulum (ER) membranes [26, 27]