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Wound and tissue repair : Accelerates tissue regeneration and wound closure by activating fibroblast migration and proliferation

J744A.1 mouse macrophage cells were collected and seeded in six well flasks at the density of 2.510 5 cells/well

The cells were then scraped and collected in 10ml of ice-cold PBS supplemented with 1x Complete protease inhibitor using a plastic cell scraper and shifted into a 50ml conical tube on ice

Glucose-6-phosphate dehydrogenase is found in all cells and is responsible for the first reaction of the pentose phosphate pathway in which glucose-6-phosphate is oxidized to 6-phosphogluconolactone with concomitant production of NADPH