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Mouse Tumor Cell Isolation, Fluorescence-Activated Cell Sorting and RNA Sequencing p48-Cre/p53F/+KrasL/+/ROSA26-LSL-YFP ( Sirt6 cKO), as well as Sirt6 WT p48-Cre/p53F/+KrasL/+/ROSA26-LSL-YFP as well as 4T1-eGFP tumor bearing mice were euthanized and entire tissues (primary tumors, livers and lungs) were minced and incubated with 1.3mg/ml collagenase (Sigma-Aldrich, C6079) on a horizontal shaker at 37 C for 30 min, vortexing every 5 min

118 In accordance with this notion, ATII was shown to increase mitochondrial ROS production in a Nox-dependent manner 119 and vice versa

TB-500 protocols use milligram-scale doses spaced across the week because the proposed cell-movement mechanism is concentration-dependent

BPC 157, or Body Protection Compound 157, is a synthetic pentadecapeptide