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Active principles L-glutathione (GSH) : tripeptide made up of cysteine, glycine, glutamic acid, it is mainly concentrated in hepatic tissue, in the pancreas, in the lungs and in the adrenal glands

caldus strains grown for 7 days without heavy metals were supplemented with CuSO 4 (final concentration: 5 mM) or ZnSO 4 (final concentration: 40 mM), respectively, incubated at 40C and shaken at 150 rpm

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Dithiodiglycolic acid [SS(CM) 2 596 mg, 3.27 mmol] was added, followed by N -hydroxysuccinimide (790 mg, 6.87 mmol), and the solution was allowed to warm to RT over 2 h