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Blastocyst formation rate: Defined as the proportion of 2PN zygotes (not just of cleaved zygotes) which are at the blastocyst stage at Day 5 (116 2 h post-insemination) (18), We employed the Gardner system to evaluate the quality of blastocysts in terms of their expansion, inner cell mass, and trophoblast development (20)

In this way, the analysis is able to reach a number needed to harm (NNH) of at least 1000

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