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Extracellular vesicles, constituted by exosomes derived from mesenchymal stem cells, can encapsulate BDNF, GDNF, and related factors, facilitating their transport across the BBB with minimal immunogenicity [241, 242]

demonstrated the use of transdermal delivery of glutathione by loading it into the hyaluronic acid microneedles.[] GLUTATHIONE POLYMER FILMS Recent research focuses on the use of GSH responsive linkers to prepare polymers which degrade upon the exposure to millimolar concentrations of GSH, and the use of these polymers to prepare particles that disassemble at these concentrations

& Brautigam, L

The answer should explain that the required volume depends on the target concentration, compound mass, and laboratory protocol design