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doi: 10.1084/jem.20050915 5 LeiGZhuangLGanB

Therefore, affinity-purified rEE2 eluates and rMCP insoluble fractions were further subjected to size exclusion chromatography (SEC) purification: For rEE2, suitable eluates were further purified using Superdex 200 increase 10/300 or 16/600 columns (Cytiva, Little Chalfont, UK) on a GE AKTA Explorer system (GE Healthcare)

In addition to the thiamin coenzyme (TPP), each dehydrogenase complex requires a niacin-containing coenzyme (NAD), a riboflavin-containing coenzyme (FAD), and lipoic acid

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