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The fact that the double-cysteine mutations only partially abolish the physiological function may imply that not all the expressed mutants have an intact disulfide bridge and that the observed conformation is only one possible inactive state on the cell surface, as supported by the EM data and MD simulations below

Samples were mixed with 10% TCA containing 0.65% 2-thiobarbituric acid (TBA) and heated at 95 C for 25 min

Total expected loss at month 12: 1522% of starting body weight depending on medication, lifestyle integration, and individual response

Pentadecapeptide means that the peptide contains 15 amino-acid residues