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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

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Post-treatment serum B12 measurements can be misleadingly high and may not accurately reflect tissue levels, so your GP will focus on symptom improvement and clinical assessment

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