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Cells were air-lifted upon confluency (=day 0 of ALI culture) by removing the apical medium and substituting the basolateral medium with PneumaCult-ALI medium containing 1% penicillin/streptomycin

Bollywood actresss sudden death reignites Indias quick-fix beauty treatment debate The death of Shefali Jariwala, 42, a user of anti-ageing injections, has raised concerns about cosmetic treatment safety and heart-related deaths among young adults Jariwala, 42, died last week at her Mumbai residence after a suspected massive cardiac arrest

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