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Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C

Beyond these neuroendocrine factors, the concentrations of macro and micronutrients and immunological components have also been shown to exhibit diurnal fluctuations

SLC7A5 overexpression weakened the protective effect of YTHDF1 knockdown, resulting in decreased cell viability, deterioration of mitochondrial function, and increased ferroptosis