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Franz diffusion cell studies comparing free GHK-Cu versus liposomal preparations document 5-fold greater dermal concentrations and 3-fold higher retention times for liposomal formulations

HO-1 and HO-2 degrade heme in the same pattern, releasing biliverdin, CO, and Fe 2+

Further development and experimentation surprisingly led to the present invention wherein with the help of selection of suitable ingredients in appropriate amounts, suitable In-vitro release pattern was obtained wherein even with lower doses, one can deliver sufficient GHK-Cu so that at least 100 micrograms, prefearably 200 micrograms and most preferably 300 micrograms of GHK-Cu remain at the end of 24 hrs

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