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Upregulation of sense gene via NAT degradation While the previously mentioned mechanisms function to upregulate the sense gene by directly acting upon the sense gene mRNA transcript, one limitation lies in the fact that uORFs only exist in approximately 50% of mammalian mRNA and within this 50%, not all are inhibitory [106, 107]

Kidney stones the carbonic anhydrase inhibition also increases the risk of nephrolithiasis

anti-PDGFR (ab5460) (1:100), anti-CC1 (OP80) (1:100) (Millipore, Darmstadt, Germany), anti-Casp8 (1:200) (NB100-56116) (Novous Biologicals, Littleton, CO) or anti-HNE (1:50) (ab48506) (Abcam, Cambridge, GB) overnight