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Glycine and glutamate are produced and used by many metabolic reactions and have relatively high cytosolic concentrations

The mineralocorticoid receptors have a more restricted expression profile throughout the body, with notably high levels of expression in the kidney and adipose tissue, although it is also expressed in certain parts of the brain (82)

The concentrations were determined by acquiring chromatograms and then using them to build a calibration curves showing the average peak areas

To confirm that the gene expression changes we observed in this study indicate DM1 disease status, we used PCA and predictive modeling