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Reusing needles is a guaranteed way to introduce contamination and also dulls the needle, which can core the rubber stopper, dropping tiny rubber particles into your solution

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Materials and methods Chemicals and culture media The PAEs used in this study, including di-methyl phthalate (DMP), di-ethyl phthalate (DEP), di-butyl phthalate (DBP) and DEHP, were purchased from Sigma-Aldrich Chemie GmbH (Hamburg, Germany), and purities were all above 99.5%