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Briefly, the lyophilized internal standards were reconstituted according to the manufacturers specifications, and the daily working solution was prepared by diluting the reconstituted internal standards 1:100 (v/v) using the extraction solution

However, there seems to be a contradictory result between adipocytes and ferroptosis, as shown by the proliferation of adipocytes despite being under ferroptosis conditions ( 2 O 2 and found that an increase in ROS enhanced adipocyte apoptosis ( In conclusion, on the basis of these studies, this review speculated that there may be two mechanisms of ferroptosis leading to obesity: (i) In the autonomic nervous system, vagus and parasympathetic nerves in some organs or tissues may be affected by ferroptosis and become dysfunctional, while the inflammatory factors released from adipocytes lead to excessive sympathetic activation and hypothalamic neuronal ferroptosis which promotes the development of obesity through a series of processes

doi: 10.1016/j.jep.2022.115427 38 SenkalCESalamaMFSniderAJAllopennaJJRanaNAKollerAet al