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Factorial experimental designs with single-compound and combined-compound treatment arms, alongside vehicle controls, allow researchers to statistically distinguish independent compound effects from any combination-specific outcomes on NAD+ pathway and mitochondrial redox endpoints, a standard approach in multi-compound cellular metabolism research.

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Bumetanide for the treatment of seizures in newborn babies with hypoxic ischaemic encephalopathy (NEMO): an open-label, dose finding, and feasibility Phase 1/2 trial