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The identification of direct targets and upstream signaling pathways still predominantly relies on computational prediction or correlative data rather than direct experimental validation, such as using chemical biology probes or genetic knockout models, leading to a high risk of false-positive claims (Wang et al., 2024)

Additionally, N-acetylcysteine increases the expression of Bcl-2 to inhibit apoptosis in KTEC cells by upregulating SIRT3 expression to exert protective effect against SAKI (Fan et al., 2024)