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Forward and reverse catalysis and product sequestration by human glutathione S-transferases in the reaction of GSH with dietary aralkyl isothiocyanates

The blots were probed with the primary antibodies specific for phosphorylated PERK and CHOP (Thermo Fischer Scientific Inc.) at 4C overnight, then the blots were rinsed 35 times for 5 min with TBST and incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG Secondary Antibody (Thermo Fischer Scientific Inc.) against the blotted target protein for 1 h at room temperature

In the normal pancreas specimen, no -SMA-positive PSCs (activated myofibroblast-like cells) were seen, however, GFAP-positive quiescent PSCs showed low expression of the GLP-1R (Figures 1 eg)