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After centrifugation with 18,000 g at 4C for 30 min, the supernatant was loaded on SPE columns (Phenomenex strata-X, 300 mg) that were preconditioned with 1 ml of methanol and 1 ml of water

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Purification of cytosolic malic enzyme from bovine brain, generation of monoclonal antibodies, and immunocytochemical localization of the enzyme in glial cells of neural primary cultures

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