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Immune cells, such as macrophages and T cells, can further modulate ferroptosis through cytokine release and redox signaling

An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm

Diagnostic & prognostic: from discovery to clinical application The persistently high incidence and mortality rates of CVD underscore an urgent need for more effective methods for early diagnosis and prognosis assessment