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After 5/10/30/60/90 minutes incubation at 37 C and under constant agitation (300 rpm) the TCEP was removed and buffer exchanged to PBS buffer (pH=7.4) with VivaSpin 500 (10 kDa MWCO) centrifugal concentrator
Regulation of ferroptosis Ferroptosis represents a distinct form of programmed cell death that diverges significantly from other established forms of cell death, including apoptosis, autophagy, and necrosis
Czapski [Journal of the American Chemical Society, 1996, vol