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The column was washed with acetonitrile for 10 min followed by a linear gradient back to the initial mixture of 30% acetonitrile with the flow rate 1 ml/min

Genet Test Mol Biomarkers (2012) 16:96873.10.1089/gtmb.2012.0091 84 SchmidtRJTancrediDJOzonoffSHansenRLHartialaJAllayeeHet alMaternal periconceptional folic acid intake and risk of autism spectrum disorders and developmental delay in the CHARGE (childhood autism risks from genetics and environment) case-control study

For individuals exposed to high levels of pollutants or those looking to support their liver health, supplementing with glutathione can be especially beneficial