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Glutamate-mediated excitotoxicity induces Ca 2+ overload

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Superoxide measurements in liver and kidney by electron paramagnetic resonance (EPR) spectroscopy Mitochondrial superoxide in liver and kidney tissue was measured by EPR spectroscopy using a mitochondrial targeted probe, 1-hydroxy-4-[2-triphenylphosphonio)-acetamido]-2,2,6,6-tetramethylpiperidine,1-Hydroxy-2,2,6,6-tetramethyl-4-[2-(triphenylphosphonio)acetamido] piperidinium dichloride (mito-TEMPO-H) (Enzo Life Sciences, Farmingdale, NY) 24,25