I feel healthier and more confident, both at work and at home
But I do uh, this idea, you know Id always thought about it, you know, totally independently that, you know, when-when Shinya Yamanaka, uh, discovered these y four transcription factors that could essentially take an old adult cell and revert it to an embryonic-like state, uh, it was like, wow
The total number of the cells was evaluated based on the following formula: where Q was the total number of sexual lineage cells observed in all disectors, h was the optical disector's height, a/f was the area of the counting frame, P was the total number of observed frames, BA was the setting of the microtome, and t was the mean of the final section thickness [36]
When the temperature was lowered to 20 C, the mixture was centrifuged at 10,000 g for 20 min, after which absorbance values were measured at 600, 450, and 532 nm