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When Humanin was applied to hepatocytes, it prevented this lipid accumulation in a concentration-dependent manner

2.8 qPCR Bacterial cells were grown in BHI to the midlog phase (OD 600nm 0.3) and exposed to 20 mM H 2 O 2 for 30 min

Approximately 30 oocytes that had extruded a first polar body were placed in calcium-free CZB medium containing 10 mM SrCl for 4 to 6 hours at 37C