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Slow, Structured Titration: Doses start low and increase gradually over weeks to minimize side effects

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sgRNA crRNA:tracrRNA sequence:AGAGGGCAAATAGcgccagg ssODN sequence: GGGAAGCCGAAGGGGAAGAGGCGGCGGCGGAGGGGCTGGCGGAGAGTCACAGAGGGCAAAAACAGGATCAGGGGCAGCAGCGGCGGCAGCAGCGGCGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCTGAcgccaggcggccgcttggccggatccaggggcggtgcagggcggccggccctgcctgcgg To test compounds for NSD2 degradation, a compound plate was prepared by diluting compounds in DMSO into cell media in a 384-deep-well plate (Revvity)