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mRNA was then chloroform-extracted and precipitated using isopropanol and washed with 75% ethanol or precipitated using the RNeasy Plus 96 Kit (Qiagen)

In one sentence: HA is a reliable, proven, surface-level hydrator that does one job well

Also levels of cleaved PARP (PolyADP ribose polymerase) 42 were detected with the appearance of a 90 kDa lower band as opposed to 113 kDa uncleaved PARP in GNE knockdown cells indicative of DNA damage (Fig