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Since the formation of stable metal-thiolate complexes precludes the direct thiol-disulfide redox potential measurements by usual electrochemical techniques, an indirect method had to be elaborated

The extracellular L-cysteine/L-cystine redox balance, and thus the synthesis of GSH, is finely regulated by the intracellular conversion of L-cystine into L-cysteine (10), and its impairment is related to oxidative stress and other pathological disorders

Under conditions of oxidative stress, excess H 2 O 2 and O 2 can increase the phosphorylation levels of RIPK1 and RIPK3, activating MLKL and inducing membrane rupture [237]