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Researchers designing comparative studies should account for: titration schedule alignment (all three compounds use gradual dose escalation), equivalent observation windows (retatrutides Phase 2 trial used 48 weeks vs semaglutides 68-week STEP trials), body weight-adjusted dosing for fair comparison, and consistent outcome endpoints

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At the beginning of an approach/retraction cycle, the p28-functionalized tip is moved toward the protein-functionalized substrate and the cantilever deflection is zero (point 1)