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The tissue powder was suspended in four volumes of cold (4C) extraction solution which contains 5% (w/v) stabilized metaphosporic acid (Acros Organics, USA) and 1 mM EDTA in 0.1% formic acid, supplemented with 1% (w/v) polyvinyl-polypyrrolidone just before use
Samples were injected automatically and separated on an analytical column Acclaim RSLC PA2, 250 2.1 mm, 2.2 m (Thermo Scientific, 074814)
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