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IV and liposomal can bypass the breakdown that may occur in digestion, which is why some recommend them more than oral supplementation

Ratios were normalized to the average of the SLC25A39 knockout cells expressing SLC25A39 cDNA

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For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)