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Trace amounts can form during production but the EU's Scientific Committee on Consumer Safety has concluded that levels at/below 10 ppm in finished products are safe (commercial products consistently fall within acceptable margins)

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In vitro models to study confinement-induced responses include micropatterned lines [151], 2D micropatterned substrates [85], uni-axial compression [152], polydimethylsiloxane [153,154,155,156]- or polyacrylamide [90]-based microchannel devices, microniches [157, 158], and natural hydrogels [148, 159]