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Briefly, yeast cells (5 10 7 ) were seeded in plates (10 cm) with 10 ml of RPMI medium and incubated for 24 h at 37C in an atmosphere of 5% CO 2 and 95% humidity

PubMed Europe PMC Scholia Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the -glutamyl cycle

Avoid: repeated freeze-thaw cycles of the reconstituted solution, vigorous shaking (direct the diluent stream down the vial wall and swirl gently rather than agitating), and prolonged light exposure

Zhu J, Xie R, Gao R, Zhao Y, Yodsanit N, Zhu M, et al