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India's average fine-particle (PM2.5) pollution level came in at nearly ten times the WHO safety guideline in IQAir's 2025 World Air Quality Report, with New Delhi named the world's most polluted capital city for an eighth year running

Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation

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