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Visual Screening of GUS-Expressing Bacteria in Transformation and Environmental Studies
Education / Demonstrations — Clear
Purpose: To quantify transcriptional activity under various experimental conditions
Kanamycin is a broad-spectrum aminoglycoside antibiotic commonly used as a selectable marker in bacterial cloning workflows
5-Fluorouracil T341 Visual Screening of GUS-Expressing Bacteria5 Fluorouracil (5 FU) is a uracil analog that has a fluorine atom on the fifth carbon position. It acts as a strong anti cancer agent where its metabolization disrupts both RNA and DNA synthesis. 5 FU metabolites, Fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine triphosphate (FUTP), imitate uracil and are incorporated into RNA strands which effectively inhibits further synthesis. Inhibition of DNA synthesis by 5 FU occurs through thymidylate
