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Levels of NNAL, which is a marker for NNK exposure, were about 0.98 micrograms per liter (ug/L) in nonsmokers, compared to 583 ug/L among smokeless tobacco users, about 218 ug/L among cigarette users and about 430 ug/L among people using both smokeless tobacco and cigarettes

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Animals receive an acute injection of either nicotine (0.4 mg/kg) or saline 78 h prior to the first diazepam drinking session