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100 L of 1 g/L sample were analysed at a flow rate of 0.3 mL/min using 0.1M potassium phosphate buffer (pH 7.0)

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Moreover, the smokeless tobacco industry is led by increasing health issues related to smoking, smoking ban regulations imposed on cigarettes, increasing demand for alternative nicotine products, heightened consumer awareness, innovations in products such as nicotine pouches, acceptance of the culture in some markets, and advertising campaigns supporting smokeless products as a less risky alternative to smoking