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A priori correlations between CBF estimates from each cluster and urine cotinine level, nicotine dependence severity (from the Brief WISDM PDM scale), and self-reported NTP recency (days since last use) were tested via bivariate Pearson correlation tests (three correlations per region

Regrettably, there is a dearth of research on social media marketing activities of the tobacco industry in Poland, therefore creating a gap in knowledge that needs to be addressed 17-19

PCR products were purified either as pooled amplicons or following agarose gel separation using QIAquick columns (Qiagen), cloned into the pJET1.2 vector (Thermo Fisher Scientific), and transformed colonies were pooled for Oxford Nanopore Technologies (ONT) sequencing