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Ussing chamber analysis On day 32 post-airlift, four replicate ALI cultures from each KO treatment (4 donors) were subjected to electrophysiological analyses in an Ussing Chamber (Physiologic Instruments) under open-circuit conditions, where intermittent short-circuit current measurements with pulsing (200 ms pulses at 5 mV) was performed to obtain resistance and conductance values

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We should note here that cohort-specific cigarette consumption varies greatly from state to state due to, in part, the differences in state-level tobacco control policies such as cigarette taxes and smoke-free air laws, as well as social norms and sociodemographic composition 58

Government of Canada Federal Excise Duty on Tobacco