why are cigarettes addictive don't smokers quit? – ASH > Action on Smoking & Health Why Is Nicotine So Addictive?

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At 80% confluency, cells were harvested using Dulbeccos phosphate buffered saline (DPBS) for washing and incubated with 0.05% trypsin EDTA/DPBS (GIBCO, Invitrogen, Carlsbad, CA) for 2 mins at 37 C to allow detachment from the culture flask

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Ventilation : Adequate ventilation, such as opening a window during smoking, can help dissipate the smoke swiftly, preventing it from deeply settling into surrounding objects and fabrics