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Coronal slices (300 m thick) containing the CeA were prepared with a Leica VT1000S (Leica Microsystems) and incubated in oxygenated (95% O 2 /5% CO 2 ) artificial CSF (aCSF) containing the following: 120 mm NaCl, 2.5 mm KCL, 5 mm EGTA, 2.0 mm CaCl 2 , 1.0 mm MgCl 2 , 1.2 mm NaH 2 PO 4 , 26 mm NaHCO 3 , 1.75 mm glucose, and 5 mm HEPES for 30 min at 37C, followed by 30-min equilibration at room temperature (RT
Leonardo DiCaprio is backed by the likes of Jonah Hill as his just-as irresponsible sidekick and Margot Robbie as his neglected trophy wife amongst others such as Rob Reiner, Kyle Chandler and Jean Dujardin
Age, another covariate in our study, was also determined to be a risk factor for osteoporosis, as in previous research 30
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