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The cell extract (90 L) thus obtained was added with 1 l of substrate, 7-ethoxyresorufin (100 M) and incubated at 37 C for 10 minutes

It is enough to imagine the effect of any changes in the organization of the grid (such as, putting Victoria on the right in the middle tier, or moving Davids bottom tier image up top) to note that the conversation which viewers are asked to simulate is structured to represent the specific stance-shift happening throughout the conversation

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In fact, the Denmark study reported a similarly small LE gap at age 65 between male ex-smokers and non-smokers [13]