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Federal data from 2019 found that more than one in four high school students had used an e-cigarette in the past 30 days, which was an increase of nearly 12 per cent over the previous year

MTT assay At 24, 48, or 72 h following the start of treatment with smoking-related stimuli as detailed above, cell viability was examined using an MTT assay according to standard protocols 40

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