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For immunofluorescent staining, after incubated with primary antibodies, the sections were incubated with fluorescent secondary antibody Goat anti-Mouse IgG 488 (A11001, Thermo Scientific), Goat anti-Mouse IgG 594 (A11005, Thermo Scientific), Goat anti-Rabbit IgG 488 (A11008, Thermo Scientific), Goat anti-Rabbit IgG 594 (A11012, Thermo Scientific) for 1h at room temperature

Clinically decompensated liver disease (jaundice or other signs of liver failure) 9

"The way I came to the interview is the way I came to the job," Jools explains

That meant that as Reed was portraying a mother on television, she was actually living through the ups and downs of parenthood in real life