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The health hazards of tobacco consumption were first brought to public attention in 1602, when an anonymous essay focused on the effects that soot and tobacco exposure had on the health of English chimney sweepers was published

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Proteomic analysis of apical secretions of chronically tobacco exposed airway cultures Apical secretions were collected and processed to identify and quantitate the proteins using mass spectrometry based proteomic analysis 52

Fourth, this study examined flavored cigars while the study by Cohen and colleagues (2021) did not include cigars in their sample [35]