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Fourth, this study is primarily descriptive and relies on proportions and chi-squared tests to compare themes across time periods, without conducting inferential or causal analysis

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We investigated with voltage-clamp experiments, effects of aqueous extracts (coffees) of unroasted and roasted coffee beans on the activity of human brain nicotinic acetylcholine receptor (nAChR) subtypes expressed in Xenopus oocytes, looking at complex brews, low molecular weight (LMW) fractions, and specific compounds present in coffee

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